Human UBE2D2 Recombinant Protein (RPPB2369)
- SKU:
- RPPB2369
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- UBE2D2
- Synonyms:
- ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
- UBC4
- Ubiquitin carrier protein D2
- Ubiquitin-conjugating enzyme E2-17 kDa 2
- Source:
- Escherichia Coli
- Uniprot:
- P62837
Description
| Product Name: | Human UBE2D2 Recombinant Protein |
| Product Code: | RPPB2369 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2D2 |
| Synonyms: | ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC4, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, UBCH5B, EC 6.3.2.19, E2(17)KB2, PUBC1, UBC4/5, UBC5B, UBCH4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The UBE2D2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM |
UBE2D2 belongs to the E2 ubiquitin-conjugating enzyme family. UBE2D2 takes part in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a SCFB-TRCP and phosphorylation dependent method.
UBE2D2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-147) and having a molecular mass of 18.9 kDa.The UBE2D2 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: cytosol; nucleoplasm; protein complex; ubiquitin ligase complex Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein autoubiquitination; protein modification process; protein polyubiquitination; protein ubiquitination; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; ubiquitin-dependent protein catabolic process |
| NCBI Summary: | Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P62837 |
| NCBI GenInfo Identifier: | 51338685 |
| NCBI Gene ID: | 7322 |
| NCBI Accession: | P62837.1 |
| UniProt Secondary Accession: | P62837,P51669, Q3MN78, Q96RP6, D3DQC9, |
| UniProt Related Accession: | P62837 |
| Molecular Weight: | 13,635 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 D2 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 D2 |
| NCBI Official Symbol: | UBE2D2 |
| NCBI Official Synonym Symbols: | UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 D2 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 D2 |
| UniProt Synonym Protein Names: | (E3-independent) E2 ubiquitin-conjugating enzyme D2 (EC:2.3.2.24 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2D2 |
| UniProt Entry Name: | UB2D2_HUMAN |
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