Human TPMT Recombinant Protein (RPPB2670)

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SKU:
RPPB2670
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
TPMT
Synonyms:
TPMT
Synonyms:
Thiopurine S-methyltransferase
Synonyms:
EC 21167
Synonyms:
Thiopurine methyltransferase
Source:
Escherichia Coli
Uniprot:
P51580
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Description

Product Name:Human TPMT Recombinant Protein
Product Code:RPPB2670
Size:20µg
Species:Human
Target:TPMT
Synonyms:TPMT, Thiopurine S-methyltransferase, EC 2.1.1.67, Thiopurine methyltransferase.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:1 mg/ml solution containing 20mM Tris 8.0, 0.2mM PMSF and 2mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGKSGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKVFKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLYLLTEK

TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.

TPMT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 245 amino acids (1-245) and having a molecular mass of 28 kDa. Thiopurine S-methyltransferase is purified by proprietary chromatographic techniques.

UniProt Protein Function:TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.1.1.67; Methyltransferase

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function:thiopurine S-methyltransferase activity

Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency

NCBI Summary:This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]
UniProt Code:P51580
NCBI GenInfo Identifier:1730006
NCBI Gene ID:7172
NCBI Accession:P51580.1
UniProt Secondary Accession:P51580,O14806, O15423, O15424, O15425, O15426, O15515 O15548, O43213, Q5VUK6, Q9UBE6, Q9UBT8,
UniProt Related Accession:P51580
Molecular Weight:245
NCBI Full Name:Thiopurine S-methyltransferase
NCBI Synonym Full Names:thiopurine S-methyltransferase
NCBI Official Symbol:TPMT  
NCBI Protein Information:thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase
UniProt Protein Name:Thiopurine S-methyltransferase
UniProt Synonym Protein Names:Thiopurine methyltransferase
UniProt Gene Name:TPMT  
UniProt Entry Name:TPMT_HUMAN
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