Human TNNI1 (Troponin I Type 1, Slow Skeletal) CLIA Kit
The Human TNNI1 (Troponin I, Type 1) Slow Skeletal CLIA Kit is a highly reliable and accurate assay designed for the precise measurement of TNNI1 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring consistent and reproducible results that are perfect for a wide range of research applications.TNNI1 is a critical protein involved in muscle contraction, specifically in slow-twitch skeletal muscle fibers.
Its levels can be indicative of muscle damage and various muscular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human TNNI1 Slow Skeletal CLIA Kit is an essential tool for researchers studying muscle physiology, muscle disorders, and potential treatments for various muscle-related conditions. Its accuracy and reliability make it a must-have for any laboratory conducting research in this area.
Product Name:
Human TNNI1 (Troponin I Type 1, Slow Skeletal) CLIA Kit
SKU:
HUES00808
Target:
Human TNNI1 (Troponin I Type 1, Slow Skeletal)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNNI1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNI1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNNI1. You can calculate the concentration of Human TNNI1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
96-109
97-110
Average (%)
102
103
105
1:4
Range (%)
98-115
97-108
98-112
Average (%)
105
103
103
1:8
Range (%)
101-111
103-116
100-118
Average (%)
106
109
108
1:16
Range (%)
90-105
88-99
91-104
Average (%)
98
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.46
74.06
296.59
43.42
78.52
283.01
Standard deviation
3.4
5.9
19.34
3.5
7.97
31.53
C V (%)
8.01
7.97
6.52
8.06
10.15
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNNI1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNI1 in samples. No significant cross-reactivity or interference between Human TNNI1 and analogues was observed.
