Human THG1L Recombinant Protein (RPPB2317)

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SKU:
RPPB2317
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
THG1L
Synonyms:
tRNA-histidine guanylyltransferase 1-like (S cerevisiae)
Synonyms:
Interphase cytoplasmic foci protein 45
Synonyms:
probable tRNA(His) guanylyltransferase
Synonyms:
induced by high glucose-1
Source:
Escherichia Coli
Uniprot:
Q9NWX6
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Description

Product Name:Human THG1L Recombinant Protein
Product Code:RPPB2317
Size:10µg
Species:Human
Target:THG1L
Synonyms:tRNA-histidine guanylyltransferase 1-like (S. cerevisiae), Interphase cytoplasmic foci protein 45, probable tRNA(His) guanylyltransferase, induced by high glucose-1, FLJ11601, FLJ20546, ICF45, IHG-1, EC 2.7.7.79, EC 2.7.7.6.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The THG1L solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAKSKFE YVRDFEADDT CLAHCWVVVR LDGRNFHRFA EKHNFAKPND SRALQLMTKC AQTVMEELED IVIAYGQSDE YSFVFKRKTN WFKRRASKFM THVASQFASS YVFYWRDYFE DQPLLYPPGF DGRVVVYPSN QTLKDYLSWR QADCHINNLY NTVFWALIQQ SGLTPVQAQG RLQGTLAADK NEILFSEFNI NYNNELPMYR KGTVLIWQKV DEVMTKEIKL PTEMEGKKMA VTRTRTKPVP LHCDIIGDAF WKEHPEILDE DS

Probable tRNA (His) guanylyltransferase (THG1L) is a member of the tRNA (His) gyanylytransferase family. THG1L which is localized to the cytoplasm also found adjacent to the nuclear membrane and expressed in various tissues, including liver and lung. THG1L is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. THG1L adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is vital for accurate recognition of the tRNA and for the fidelity of protein synthesis. Upon DNA damage, THG1L is phosphorylated, presumably by ATM or ATR.

THG1L Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (30-298) and having a molecular mass of 34.0 kDa.THG1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ICF45: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Belongs to the tRNA(His) guanylyltransferase family.Protein type: EC 2.7.7.79; Cell cycle regulation; Mitochondrial; TransferaseChromosomal Location of Human Ortholog: 5q33.3Cellular Component: mitochondrion; cytoplasmMolecular Function: identical protein binding; tRNA guanylyltransferase activity; GTP binding; magnesium ion binding; ATP binding; tRNA bindingBiological Process: tRNA processing; tRNA modification; protein homotetramerization
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene is a mitochondrial protein that is induced by high levels of glucose and is associated with diabetic nephropathy. The encoded protein appears to increase mitochondrial biogenesis, which could lead to renal fibrosis. Another function of this protein is that of a guanyltransferase, adding GMP to the 5' end of tRNA(His). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]
UniProt Code:Q9NWX6
NCBI GenInfo Identifier:146325755
NCBI Gene ID:54974
NCBI Accession:Q9NWX6.2
UniProt Secondary Accession:Q9NWX6
UniProt Related Accession:Q9NWX6
Molecular Weight:
NCBI Full Name:Probable tRNA(His) guanylyltransferase
NCBI Synonym Full Names:tRNA-histidine guanylyltransferase 1 like
NCBI Official Symbol:THG1L  
NCBI Official Synonym Symbols:ICF45; IHG-1; hTHG1  
NCBI Protein Information:probable tRNA(His) guanylyltransferase
UniProt Protein Name:Probable tRNA(His) guanylyltransferase
UniProt Synonym Protein Names:Interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase
Protein Family:
UniProt Gene Name:THG1L  
UniProt Entry Name:THG1_HUMAN
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