The Human TACR2 (Tachykinin Receptor 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TACR2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for research applications.Tachykinin Receptor 2, also known as Neurokinin 2 receptor, is a key protein involved in mediating the effects of tachykinins, neuropeptides that play a role in inflammatory and immune responses. Dysregulation of TACR2 has been associated with various conditions, including pain, asthma, and emotional disorders, making it a valuable biomarker for studying these diseases.
By utilizing the Human TACR2 CLIA Kit, researchers can accurately quantify TACR2 levels in biological samples, aiding in the understanding of its role in disease pathology and potentially identifying new therapeutic targets. Trust in this kit to provide the precise measurements needed for cutting-edge research in the field of neurobiology and beyond.
Product Name:
Human TACR2 (Tachykinin Receptor 2) CLIA Kit
SKU:
HUES01071
Target:
Human TACR2 (Tachykinin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TACR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TACR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TACR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TACR2. You can calculate the concentration of Human TACR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
93-108
92-109
Average (%)
108
99
100
1:4
Range (%)
88-99
93-110
99-114
Average (%)
93
100
106
1:8
Range (%)
98-115
98-111
86-100
Average (%)
105
103
93
1:16
Range (%)
101-115
88-102
101-114
Average (%)
107
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
98-112
105
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.62
454.97
1408.6
202.4
413.89
1459.01
Standard deviation
18.86
52.87
128.04
18.66
29.01
166.18
C V (%)
8.51
11.62
9.09
9.22
7.01
11.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TACR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TACR2 in samples. No significant cross-reactivity or interference between Human TACR2 and analogues was observed.
