The Human Survivin ELISA Kit is specifically designed for the precise measurement of survivin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Survivin is a key protein that plays a vital role in regulating cell division and preventing apoptosis. It is closely associated with cancer development and progression, making it a valuable biomarker for studying cancer biology and developing new treatment strategies.
With the Human Survivin ELISA Kit, researchers can confidently analyze survivin levels in various biological samples, enabling them to gain insights into the role of survivin in cancer and other diseases. This kit provides a reliable tool for advancing research in oncology and other fields related to cell proliferation and apoptosis.
Product Name:
Human Surv (Survivin) ELISA Kit
SKU:
HUES02594
Target:
Human Surv (Survivin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Surv. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Surv and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Surv, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Surv. You can calculate the concentration of Human Surv in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
87-101
94-111
Average (%)
104
94
102
1:4
Range (%)
88-104
80-91
86-96
Average (%)
95
85
91
1:8
Range (%)
93-104
79-91
83-96
Average (%)
98
85
90
1:16
Range (%)
93-108
84-94
86-100
Average (%)
100
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.5
294.17
752.93
96.1
280.43
759.27
Standard deviation
5.37
12.77
28.61
4.85
12.51
24.07
C V (%)
5.45
4.34
3.8
5.05
4.46
3.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Surv concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Surv in samples. No significant cross-reactivity or interference between Human Surv and analogues was observed.
