Mothers against decapentaplegic homolog 2(SMAD2) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD2 is contains 1 MH1 (MAD homology 1) domain and1 MH2 (MAD homology 2) domain. It is expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. It binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. It may act as a tumor suppressor in colorectal carcinoma. And SMAD2 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Product Name:
Human SMAD2 Recombinant Protein (RPES4805)
Product Code:
RPES4805
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Mothers against decapentaplegic homolog 2, MAD homolog 2, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, Hsmad2, SMAD2, MADH2, MADR2
Accession:
Q15796
Sequence:
Ser2-Ser467
Fusion tag:
N-6His-Flag
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Mothers Against Decapentaplegic Homolog 2 is produced by our E.coli expression system and the target gene encoding Ser2-Ser467 is expressed with a 6His, Flag tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
54.6 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,500mM NaCl, pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
