Human SIRT5 Recombinant Protein (RPPB4694)

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SKU:
RPPB4694
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
SIRT5
Synonyms:
Sirtuin 5
Synonyms:
NAD-Dependent Lysine Demalonylase AndDesuccinylase Sirtuin-5
Synonyms:
Mitochondria
Synonyms:
NAD-Dependent Protein Deacylase Sirtuin-5Mitochondrial
Source:
Escherichia Coli
Uniprot:
Q9NXA8
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Description

Product Name:Human SIRT5 Recombinant Protein
Product Code:RPPB4694
Size:20µg
Species:Human
Target:SIRT5
Synonyms:Sirtuin 5, NAD-Dependent Lysine Demalonylase AndDesuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae)Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:SIRT5 protein solution (0.5mg/ml) containing 20mMTris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKWQAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAGTKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHVVWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQGPCGTTLPEA LACHENETVS

SIRT5 belongs to the Sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members hold a sirtuin core domain and grouped into four classes. The protein encoded by this gene is included in class III of the sirtuin family. Human sirtuins functions were not yet determined; still, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies propose that human sirtuin could operate as intracellular regulatory protein with mono-ADP-ribosyltransferase activity. 

SIRT5 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain topological domain containing 300 aminoacids (34-310 a.a) and having a molecular mass of 32.5kDa. SIRT5 is fused to a 23amino acid His-tag at N-terminus & purified by proprietary chromatographictechniques.

UniProt Protein Function:SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function:zinc ion binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; protein amino acid deacetylation; chromatin silencing

NCBI Summary:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]
UniProt Code:Q9NXA8
NCBI GenInfo Identifier:38258652
NCBI Gene ID:23408
NCBI Accession:Q9NXA8.2
UniProt Secondary Accession:Q9NXA8,Q5T294, Q5T295, Q9Y6E6, B4DFM4, B4DYJ5, F5H5Z9
UniProt Related Accession:Q9NXA8
Molecular Weight:310
NCBI Full Name:NAD-dependent protein deacylase sirtuin-5, mitochondrial
NCBI Synonym Full Names:sirtuin 5
NCBI Official Symbol:SIRT5  
NCBI Official Synonym Symbols:SIR2L5  
NCBI Protein Information:NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIRT5; sir2-like 5; sirtuin type 5; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial
UniProt Protein Name:NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Synonym Protein Names:Regulatory protein SIR2 homolog 5; SIR2-like protein 5
Protein Family:NAD-dependent protein deacylase sirtuin
UniProt Gene Name:SIRT5  
UniProt Entry Name:SIR5_HUMAN
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