Human SH2D1B Recombinant Protein (RPPB4671)
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주문- SKU:
- RPPB4671
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- SH2D1B
- Synonyms:
- SH2 Domain Containing 1B
- Synonyms:
- EWS/FLI1-Activated Transcript 2
- Synonyms:
- EAT-2
- Synonyms:
- EAT2
- Source:
- Sf9 Insect cells
- Uniprot:
- O14796
Description
Product Name: | Human SH2D1B Recombinant Protein |
Product Code: | RPPB4671 |
Size: | 10µg |
Species: | Human |
Target: | SH2D1B |
Synonyms: | SH2 Domain Containing 1B, EWS/FLI1-Activated Transcript 2, EAT-2, EAT2, SH2 Domain-Containing Molecule EAT2, SH2 Domain-Containing Protein 1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2. |
Source: | Sf9 Insect cells |
Physical Appearance: | Sterile filtered colorless solution. |
Formulation: | SH2D1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 20% glycerol. |
Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
Purity: | Greater than 90.0% as determined by SDS-PAGE. |
Amino Acid Sequence: | ADPMDLPYYH GRLTKQDCET LLLKEGVDGN FLLRDSESIP GVLCLCVSFK NIVYTYRIFR EKHGYYRIQT AEGSPKQVFP SLKELISKFE KPNQGMVVHL LKPIKRTSPS LRWRGLKLEL ETFVNSNSDY VDVLPHHHHH H |
SH2 domain-containing protein 1B, also known as SH2D1B activates NK cells. SH2D1B participates in controlling signal transduction through at least 4 receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. Two isoforms of the human SH2D1B are produced by alternative splicing. Superior Mesenteric Artery Syndrome is one of the diseases which is associated with SH2D1B.
SH2D1B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (1-132a.a.) and having a molecular mass of 16.4kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SH2D1B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
UniProt Protein Function: | Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling. In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation. |
NCBI Summary: | By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008] |
UniProt Code: | O14796 |
NCBI GenInfo Identifier: | 23503051 |
NCBI Gene ID: | 117157 |
NCBI Accession: | O14796.2 |
UniProt Secondary Accession: | O14796,Q5T0L1, Q8NI18, Q969K9, B2RBN6, |
UniProt Related Accession: | O14796 |
Molecular Weight: | 9,024 Da |
NCBI Full Name: | SH2 domain-containing protein 1B |
NCBI Synonym Full Names: | SH2 domain containing 1B |
NCBI Official Symbol: | SH2D1B |
NCBI Official Synonym Symbols: | EAT2 |
NCBI Protein Information: | SH2 domain-containing protein 1B |
UniProt Protein Name: | SH2 domain-containing protein 1B |
UniProt Synonym Protein Names: | EWS/FLI1-activated transcript 2; EAT-2 |
Protein Family: | SH2 domain-containing protein |
UniProt Gene Name: | SH2D1B |