Human SGK1 Recombinant Protein (RPPB2647)

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SKU:
RPPB2647
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
SGK1
Synonyms:
Serine/threonine-protein kinase Sgk1
Synonyms:
Serum/glucocorticoid-regulated kinase 1
Synonyms:
SGK1
Synonyms:
SGK
Source:
Escherichia Coli
Uniprot:
O00141
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Description

Product Name:Human SGK1 Recombinant Protein
Product Code:RPPB2647
Size:10µg
Species:Human
Target:SGK1
Synonyms:Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK1, SGK.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:SGK1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.2M NaCl, 2mM DTT and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL

Serum-and glucocorticoid-regulated kinase (SGK1) is a serine/threonine protein kinase and a member of the “AGC” subfamily, which includes protein kinases A, G, and C. The SGK1 protein has an imperative role in activating specific potassium, sodium and chloride channels, suggesting a participation in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK1 is activated in vitro by PDK-1 (3-phosphoinositide-dependent protein kinase-1) and in vivo in reaction to signals which activate phosphatidylinositol (PI) 3-kinase.

SGK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (60-431 a.a.) and having a molecular mass of 44.5kDa.SGK1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.
UniProt Protein Details:

Protein type:Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Motility/polarity/chemotaxis; Kinase, protein; AGC group; SGK family

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; mitochondrion; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function:3-phosphoinositide-dependent protein kinase binding; ATP binding; calcium channel regulator activity; chloride channel regulator activity; cofactor binding; potassium channel regulator activity; protein binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; sodium channel regulator activity; tau protein binding

Biological Process: apoptosis; cellular sodium ion homeostasis; glucocorticoid mediated signaling; long-term memory; microtubule depolymerization; negative regulation of apoptosis; negative regulation of microtubule polymerization; neurite morphogenesis; peptidyl-serine phosphorylation; positive regulation of cell growth; positive regulation of dendrite morphogenesis; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of apoptosis; regulation of blood pressure; regulation of catalytic activity; regulation of cell cycle; regulation of cell growth; regulation of cell migration; regulation of cell proliferation; regulation of transcription factor activity; response to DNA damage stimulus; response to stress; sodium ion transport; transmembrane transport; visual learning

NCBI Summary:This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
UniProt Code:O00141
NCBI GenInfo Identifier:90185131
NCBI Gene ID:6446
NCBI Accession:O00141.2
UniProt Secondary Accession:O00141,Q5TCN2, Q5TCN3, Q5TCN4, Q5VY65, Q9UN56, B7UUP7 B7UUP8, B7UUP9, B7Z5B2, E1P583,
UniProt Related Accession:O00141
Molecular Weight:
NCBI Full Name:Serine/threonine-protein kinase Sgk1
NCBI Synonym Full Names:serum/glucocorticoid regulated kinase 1
NCBI Official Symbol:SGK1  
NCBI Official Synonym Symbols:SGK  
NCBI Protein Information:serine/threonine-protein kinase Sgk1
UniProt Protein Name:Serine/threonine-protein kinase Sgk1
UniProt Synonym Protein Names:Serum/glucocorticoid-regulated kinase 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:SGK1  
UniProt Entry Name:SGK1_HUMAN
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