The Human SERT (Serotonin Transporter) ELISA Kit is specifically designed for the precise measurement of serotonin transporter levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.The serotonin transporter is a key protein responsible for regulating serotonin levels in the brain, playing a crucial role in mood regulation, behavior, and mental health.
Dysregulation of the serotonin transporter has been linked to various psychiatric disorders such as depression, anxiety, and OCD, making it an important biomarker for studying these conditions and developing potential treatments.With the Human SERT ELISA Kit, researchers can easily and accurately measure serotonin transporter levels in human samples, providing valuable insights into serotonin regulation and its implications for mental health and other related conditions.
Product Name:
Human SERT (Serotonin Transporter ) ELISA Kit
SKU:
HUES03083
Target:
Human SERT (Serotonin Transporter )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SERT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SERT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SERT. You can calculate the concentration of Human SERT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
85-97
94-106
Average (%)
98
92
100
1:4
Range (%)
86-102
83-95
83-96
Average (%)
93
89
88
1:8
Range (%)
88-104
80-92
86-99
Average (%)
95
87
91
1:16
Range (%)
92-107
86-97
88-101
Average (%)
100
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.91
91.49
355.37
57.56
92.99
374.91
Standard deviation
3.05
4.66
10.8
3.4
4.42
20.21
C V (%)
5.76
5.09
3.04
5.91
4.75
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SERT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SERT in samples. No significant cross-reactivity or interference between Human SERT and analogues was observed.
