Human SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
The Human SDF-1 (Stromal Cell-Derived Factor-1) ELISA Kit is specifically designed to accurately detect levels of SDF-1 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.SDF-1 is a critical chemokine involved in various biological processes, including stem cell homing, immune cell trafficking, and tissue repair. Its ability to attract stem cells and promote their migration to damaged tissues makes it a key player in regenerative medicine and tissue engineering.
Additionally, SDF-1 has been implicated in diseases such as cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a biomarker for disease research and therapeutic development.Overall, the Human SDF-1 ELISA Kit provides a valuable tool for researchers seeking to study the role of SDF-1 in health and disease, enabling them to accurately measure its levels in biological samples and further explore its potential therapeutic applications.
Product Name:
Human SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
SKU:
HUES01339
Target:
Human SDF-1 (Stromal Cell Derived Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-1/CXCL12. You can calculate the concentration of Human SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
86-99
95-111
Average (%)
90
91
103
1:4
Range (%)
95-109
89-103
92-106
Average (%)
103
94
100
1:8
Range (%)
95-110
81-94
93-107
Average (%)
102
87
100
1:16
Range (%)
96-108
83-93
93-108
Average (%)
102
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.6
0.5
1.5
3.6
Standard deviation
0.03
0.07
0.19
0.03
0.09
0.14
C V (%)
6
5.0
5.28
6.0
6.0
3.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1/CXCL12 and analogues was observed.
