Human SC5b9 (soluble terminal complement complex) ELISA Kit
The Human SC5b-9 (Soluble Terminal Complement Complex) ELISA Kit is specifically designed for the precise detection of soluble SC5b-9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and replicable results, making it a valuable tool for various research applications.The soluble terminal complement complex SC5b-9 is a key component of the complement system, playing a critical role in immune response and inflammation. Abnormal levels of SC5b-9 have been associated with various autoimmune diseases, infections, and inflammatory conditions, making it a vital biomarker for understanding these diseases and developing potential treatments.
With the Human SC5b-9 ELISA Kit, researchers can accurately measure SC5b-9 levels in biological samples, advancing our understanding of the complement system and its role in health and disease. This kit is an essential resource for scientists seeking to explore the mechanisms of immune response and discover new therapeutic targets.
Product Name:
Human SC5b9 (soluble terminal complement complex) ELISA Kit
SKU:
HUES03247
Target:
Human SC5b9 (soluble terminal complement complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sC5b-9. You can calculate the concentration of Human sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
98-111
91-105
Average (%)
104
105
97
1:4
Range (%)
92-107
87-102
88-102
Average (%)
99
93
93
1:8
Range (%)
89-106
84-96
88-98
Average (%)
97
89
93
1:16
Range (%)
95-106
81-90
82-92
Average (%)
100
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.53
183.12
990.39
100.54
172.48
1055.72
Standard deviation
5.84
8.66
37.24
5.93
10.23
35.79
C V (%)
6.38
4.73
3.76
5.9
5.93
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sC5b-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sC5b-9 in samples. No significant cross-reactivity or interference between Human sC5b-9 and analogues was observed.
