Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit
The Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit is a highly reliable assay designed for the precise quantification of S100A13 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for various research purposes.S100A13 is a key calcium-binding protein that plays a pivotal role in cellular signaling and regulation. Its involvement in processes such as cell growth, differentiation, and apoptosis makes it a crucial player in physiological and pathological conditions.
Elevated levels of S100A13 have been associated with cancer progression, inflammation, and neurodegenerative diseases, underscoring its potential as a biomarker for disease diagnosis and prognosis.By utilizing the Human S100A13 CLIA Kit, researchers can gain valuable insights into the role of S100A13 in disease pathogenesis and progression, paving the way for the development of innovative therapeutic strategies. With its user-friendly format and high performance characteristics, this kit is an essential tool for advancing our understanding of S100A13 biology and its implications in human health.
Product Name:
Human S100A13 (S100 Calcium Binding Protein A13) CLIA Kit
SKU:
HUES00746
Target:
Human S100A13 (S100 Calcium Binding Protein A13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A13. You can calculate the concentration of Human S100A13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-113
95-107
96-111
Average (%)
103
101
104
1:4
Range (%)
92-106
88-102
86-101
Average (%)
99
93
92
1:8
Range (%)
99-112
90-107
89-103
Average (%)
106
97
95
1:16
Range (%)
86-99
86-97
98-108
Average (%)
92
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.64
482.7
1505.11
212.7
479.76
1473.53
Standard deviation
25.33
52.42
143.29
20.63
52.82
92.39
C V (%)
11.8
10.86
9.52
9.7
11.01
6.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A13 in samples. No significant cross-reactivity or interference between Human S100A13 and analogues was observed.
