Human RNF34 Recombinant Protein (RPPB4480)
- SKU:
- RPPB4480
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- RNF34
- Synonyms:
- Ring Finger Protein 34
- E3 Ubiquitin Protein Ligase
- RINGFinger Protein 34
- Caspase Regulator CARP1
- Source:
- Escherichia Coli
- Uniprot:
- Q969K3
Description
| Product Name: | Human RNF34 Recombinant Protein |
| Product Code: | RPPB4480 |
| Size: | 20µg |
| Species: | Human |
| Target: | RNF34 |
| Synonyms: | Ring Finger Protein 34, E3 Ubiquitin Protein Ligase, RINGFinger Protein 34, Caspase Regulator CARP1, Caspases-8 And -10-Associated RINGFinger Protein 1, FYVE-RING Finger Protein Momo, Human RING Finger HomologousTo Inhibitor Of Apoptosis Protein, CARP-1, hRFI, RING Finger Protein RIFF, RFI,CARP1, RIF, RIFF, E3 Ubiquitin-Protein Ligase RNF34, EC 6.3.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | RNF34 protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGS MRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPEFSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLMRLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAPSATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLSDLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCVLLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS |
RingFinger Protein 34 (RNF34) has E3 ubiquitin-protein ligase activity. RNF34 has aRINF finger, a motif recognized to be involved in protein-protein and protein-DNAinteractions. RNF34 regulates the levels of CASP8 and CASP10 by targeting themfor proteasomal degradation. In addition, RNF34 protects cells againstapoptosis induced by TNF. RNF34 also binds phosphatidylinositol 5-phosphateandphosphatidylinositol 3-phosphate. Alternatively splicing results in multipletranscript variants encoding distinct isoforms.
RNF34 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373a.a) and having a molecular mass of 44.2kDa.RNF34 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.- Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane Molecular Function:p53 binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process |
| NCBI Summary: | The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q969K3 |
| NCBI GenInfo Identifier: | 74760679 |
| NCBI Gene ID: | 80196 |
| NCBI Accession: | Q969K3.1 |
| UniProt Secondary Accession: | Q969K3,Q8NG47, Q9H6W8, B7Z933, |
| UniProt Related Accession: | Q969K3 |
| Molecular Weight: | 41,797 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF34 |
| NCBI Synonym Full Names: | ring finger protein 34 |
| NCBI Official Symbol: | RNF34 |
| NCBI Official Synonym Symbols: | RFI; RIF; RIFF; hRFI; CARP1; CARP-1 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF34 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF34 |
| UniProt Synonym Protein Names: | Caspase regulator CARP1 |
| UniProt Gene Name: | RNF34 |
| UniProt Entry Name: | RNF34_HUMAN |
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