The Human RLN2 (Relaxin-2) ELISA Kit is specifically designed for the accurate measurement of relaxin-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results, making it an invaluable tool for various research purposes.Relaxin-2 is a key hormone that plays a crucial role in various physiological processes, including reproduction, cardiovascular function, and tissue remodeling.
Dysregulation of relaxin-2 has been associated with conditions such as heart failure, fibrosis, and inflammation, highlighting its significance as a potential biomarker for studying and possibly treating these diseases.With its advanced technology and reliable performance, the Human RLN2 ELISA Kit is an indispensable resource for researchers investigating the role of relaxin-2 in health and disease.
Product Name:
Human RLN2 (Relaxin-2) ELISA Kit
SKU:
HUES02592
Target:
Human RLN2 (Relaxin-2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RLN2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RLN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RLN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RLN2. You can calculate the concentration of Human RLN2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
94-108
89-103
Average (%)
105
99
94
1:4
Range (%)
90-104
84-98
84-94
Average (%)
95
91
89
1:8
Range (%)
89-99
85-99
82-95
Average (%)
94
92
89
1:16
Range (%)
94-104
84-98
85-97
Average (%)
99
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
89-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.4
43.71
244.95
27.29
41.78
265.59
Standard deviation
1.61
2.57
9.46
1.54
2.34
14.24
C V (%)
6.34
5.88
3.86
5.64
5.6
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RLN2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
