The Human RETN (Resistin) ELISA Kit is specifically designed for the precise measurement of resistin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Resistin is a key adipokine that plays a critical role in regulating insulin sensitivity, inflammation, and metabolism. Elevated resistin levels have been linked to various metabolic disorders, including obesity, insulin resistance, and type 2 diabetes.
As such, this ELISA kit is an invaluable tool for investigating the role of resistin in these conditions and exploring potential therapeutic interventions.Overall, the Human RETN (Resistin) ELISA Kit provides researchers with a reliable and efficient method for quantifying resistin levels in various biological samples, facilitating in-depth studies on its physiological and pathological functions.
Product Name:
Human RETN (Resistin) ELISA Kit
SKU:
HUES02284
Target:
Human RETN (Resistin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RETN. You can calculate the concentration of Human RETN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
94-108
88-105
Average (%)
96
101
96
1:4
Range (%)
95-107
85-97
97-111
Average (%)
102
91
104
1:8
Range (%)
95-109
82-92
95-109
Average (%)
101
87
103
1:16
Range (%)
94-111
88-101
97-110
Average (%)
102
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.6
212.0
977.1
112.0
217.5
915.3
Standard deviation
7.1
11.9
44.9
7.7
12.2
33.9
C V (%)
6.79
5.61
4.6
6.88
5.61
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
