Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.
Product Name:
Human REG4/RELP Recombinant Protein (RPES3096)
Product Code:
RPES3096
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Regenerating islet-derived protein 4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, GISP, RELP, REG4,GISP,REG-IV
Accession:
NP_001152824.1
Sequence:
Met 1-Pro 158
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions.The ED50 for this effect is typically 4-20 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human REG4 (NP_001152824.1) (Met 1-Pro 158) was expressed, with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.4 kDa
AP Mol Mass:
18 and 22 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.