Human ProGRP (Pro-Gastrin Releasing Peptide) ELISA Kit
The Human ProGRP (Pro Gastrin Releasing Peptide) ELISA Kit is specifically designed for the precise measurement of ProGRP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.ProGRP is a vital biomarker associated with various cancers, particularly small cell lung cancer and neuroendocrine tumors. Its detection can aid in early diagnosis, prognosis, and monitoring of these diseases, making it a valuable tool in research and clinical settings.
With reliable performance and easy-to-use protocols, the Human ProGRP ELISA Kit from AssayGenie is an essential tool for studying the role of ProGRP in cancer biology and developing potential diagnostic and therapeutic strategies. Suitable for both basic research and clinical applications, this kit is a valuable asset for any laboratory focused on cancer research and biomarker discovery.
Product Name:
Human ProGRP (Pro-Gastrin Releasing Peptide) ELISA Kit
SKU:
HUES02976
Target:
Human ProGRP (Pro-Gastrin Releasing Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ProGRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ProGRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ProGRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ProGRP. You can calculate the concentration of Human ProGRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
90-104
88-102
Average (%)
93
96
95
1:4
Range (%)
93-108
87-99
83-96
Average (%)
99
92
88
1:8
Range (%)
90-102
85-96
87-98
Average (%)
95
91
93
1:16
Range (%)
92-104
83-92
86-98
Average (%)
97
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
83-98
90
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.69
96.17
386.41
49.51
105.42
404.23
Standard deviation
2.73
4.85
18.08
2.56
5.82
16.45
C V (%)
5.28
5.04
4.68
5.17
5.52
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ProGRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ProGRP in samples. No significant cross-reactivity or interference between Human ProGRP and analogues was observed.
