Human POLR3H Recombinant Protein (RPPB4274)

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SKU:
RPPB4274
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
POLR3H
Synonyms:
DNA-directed RNA polymerase III subunit RPC8
Synonyms:
RNA polymerase III subunit C8
Synonyms:
DNA-directed RNA polymerase III subunit H
Synonyms:
RNA polymerase III subunit 229 kDa subunit
Source:
Escherichia Coli
Uniprot:
Q9Y535
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Description

Product Name:Human POLR3H Recombinant Protein
Product Code:RPPB4274
Size:20µg
Species:Human
Target:POLR3H
Synonyms:DNA-directed RNA polymerase III subunit RPC8, RNA polymerase III subunit C8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9 kDa subunit, RPC22.9, POLR3H, KIAA1665, RPC8.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:POLR3H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASHTKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN

Polymerase (RNA) III (DNA directed) Polypeptide H(POLR3H) is a member of the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA utilizing the 4 ribonucleoside triphosphates as substrates. POLR3H has a central role in sensing and curbing infection by intracellular bacteria and DNA viruses. POLR3H functions as nuclear and cytosolic DNA sensor which is involved in innate immune response.

POLR3H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a.) and having a molecular mass of 25.3kDa (Molecular size on SDS-PAGE will appear higher).POLR3H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:POLR3H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: centrosome; cytosol; DNA-directed RNA polymerase III complex; intracellular membrane-bound organelle; nucleoplasmMolecular Function: DNA binding; DNA-directed RNA polymerase activityBiological Process: defense response to virus; gene expression; innate immune response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q9Y535
NCBI GenInfo Identifier:29428071
NCBI Gene ID:171568
NCBI Accession:Q9Y535.1
UniProt Secondary Accession:Q9Y535,Q5M7Y8, Q96AE3, Q9BY95, B0QYH9
UniProt Related Accession:Q9Y535
Molecular Weight:19,658 Da
NCBI Full Name:DNA-directed RNA polymerase III subunit RPC8
NCBI Synonym Full Names:polymerase (RNA) III subunit H
NCBI Official Symbol:POLR3H  
NCBI Official Synonym Symbols:RPC8; RPC22.9  
NCBI Protein Information:DNA-directed RNA polymerase III subunit RPC8
UniProt Protein Name:DNA-directed RNA polymerase III subunit RPC8
UniProt Synonym Protein Names:DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9
Protein Family:DNA-directed RNA polymerase
UniProt Gene Name:POLR3H  
UniProt Entry Name:RPC8_HUMAN
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