The Human Procalcitonin (PCT) ELISA Kit is specifically designed for the precise measurement of procalcitonin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for consistent and accurate results, making it suitable for various research purposes.Procalcitonin is a vital marker for detecting bacterial infections and sepsis, as its levels significantly rise in response to these conditions.
Monitoring procalcitonin levels can aid in the early diagnosis and management of infections, guiding antibiotic therapy decisions and improving patient outcomes.Overall, the Human Procalcitonin (PCT) ELISA Kit is an invaluable tool for studying infectious diseases, sepsis, and inflammatory conditions, providing researchers with a reliable method for assessing procalcitonin levels in biological samples.
Product Name:
Human PCT (Procalcitonin) ELISA Kit
SKU:
HUES02515
Target:
Human PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
85-98
94-106
Average (%)
105
92
100
1:4
Range (%)
96-114
82-97
98-109
Average (%)
104
89
104
1:8
Range (%)
97-109
82-94
98-111
Average (%)
102
88
104
1:16
Range (%)
98-113
83-97
97-111
Average (%)
106
89
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.1
188.2
921.0
104.5
190.1
877.0
Standard deviation
7.2
8.7
45.1
6.5
8.2
45.6
C V (%)
6.98
4.62
4.9
6.22
4.31
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCT in samples. No significant cross-reactivity or interference between Human PCT and analogues was observed.
