Human PCK1 Recombinant Protein (RPPB5841)
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주문- SKU:
- RPPB5841
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PCK1
- Synonyms:
- Phosphoenolpyruvate carboxykinase 1 (soluble)
- Synonyms:
- PEPCK-C
- Synonyms:
- PEPCK1
- Synonyms:
- PEP carboxykinase
- Source:
- Escherichia Coli
- Uniprot:
- P35558
Description
| Product Name: | Human PCK1 Recombinant Protein |
| Product Code: | RPPB5841 |
| Size: | 10µg |
| Species: | Human |
| Target: | PCK1 |
| Synonyms: | Phosphoenolpyruvate carboxykinase 1 (soluble), PEPCK-C, PEPCK1, PEP carboxykinase, phosphoenolpyruvate carboxykinase cytosolic [GTP], EC 4.1.1.32. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PCK1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM |
PCK1 is a central regulating point for the regulation of gluconeogenesis. PCK1 has a vital part in this process by stimulating hepatic glucose production. The expression of PCK1 is controlled by cAMP, glucocorticoids, diet etc. Modulation of the signals controlling PCK1 levels offers a possible therapeutic tactic to the treatment of Diabetes and subsequently obesity.
PCK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 646 amino acids (1-622) and having a molecular mass of 71.7kDa.PCK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. |
| UniProt Protein Details: | Protein type:Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - pyruvate; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.1.32 Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: cytoplasm; cytosol Molecular Function:GDP binding; GTP binding; carboxylic acid binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; magnesium ion binding Biological Process: oxaloacetate metabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; response to activity; drug metabolic process; glucose homeostasis; internal protein amino acid acetylation; glycerol biosynthetic process from pyruvate; response to insulin stimulus; gluconeogenesis Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic |
| NCBI Summary: | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35558 |
| NCBI GenInfo Identifier: | 93138710 |
| NCBI Gene ID: | 5105 |
| NCBI Accession: | P35558.3 |
| UniProt Secondary Accession: | P35558,Q8TCA3, Q9UJD2, A8K437, B4DT64, |
| UniProt Related Accession: | P35558 |
| Molecular Weight: | 622 |
| NCBI Full Name: | Phosphoenolpyruvate carboxykinase, cytosolic |
| NCBI Synonym Full Names: | phosphoenolpyruvate carboxykinase 1 (soluble) |
| NCBI Official Symbol: | PCK1 |
| NCBI Official Synonym Symbols: | PEPCK1; PEPCKC; PEPCK-C |
| NCBI Protein Information: | phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase |
| UniProt Protein Name: | Phosphoenolpyruvate carboxykinase, cytosolic [GTP] |
| Protein Family: | Protein kinase |
| UniProt Gene Name: | PCK1 |
| UniProt Entry Name: | PCKGC_HUMAN |
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