The Human OC/BGP (Osteocalcin) ELISA Kit is specifically designed for the precise measurement of osteocalcin levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.Osteocalcin, also known as Bone Gla Protein (BGP), is a key protein involved in bone formation and regulation. It is essential for maintaining bone health and plays a crucial role in conditions such as osteoporosis, bone fractures, and other bone-related diseases.
By measuring osteocalcin levels, researchers can gain valuable insights into bone metabolism and assess bone health status.With its advanced technology and rigorous validation process, the Human OC/BGP (Osteocalcin) ELISA Kit is a valuable tool for studying bone physiology, investigating bone disorders, and developing potential therapies for improving bone health. Trust in this reliable kit to deliver precise and accurate results for your research needs.
Product Name:
Human OC/BGP (Osteocalcin) ELISA Kit
SKU:
HUES02389
Target:
Human OC/BGP (Osteocalcin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OC/BGP. You can calculate the concentration of Human OC/BGP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
91-108
91-105
Average (%)
94
98
97
1:4
Range (%)
94-109
80-95
95-108
Average (%)
101
87
102
1:8
Range (%)
101-117
83-95
98-112
Average (%)
107
88
104
1:16
Range (%)
99-113
83-97
90-102
Average (%)
107
89
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
93-109
99
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.7
9.2
32.9
3.8
9.5
36.1
Standard deviation
0.2
0.4
1.6
0.3
0.5
1.3
C V (%)
5.41
4.35
4.86
7.89
5.26
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OC/BGP in samples. No significant cross-reactivity or interference between Human OC/BGP and analogues was observed.
