Human NUDT21 Recombinant Protein (RPPB2050)
- SKU:
- RPPB2050
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- NUDT21
- Synonyms:
- Cleavage and polyadenylation specificity factor subunit 5
- Cleavage and polyadenylation specificity factor 25 kDa subunit
- CFIm25
- CPSF 25 kDa subunit
- Source:
- Escherichia Coli
- Uniprot:
- O43809
Description
| Product Name: | Human NUDT21 Recombinant Protein |
| Product Code: | RPPB2050 |
| Size: | 20µg |
| Species: | Human |
| Target: | NUDT21 |
| Synonyms: | Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit, NUDT21, CFIM25, CPSF25, CPSF5, DKFZp686H1588. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NUDT21 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN |
NUDT21 belongs to the Nudix hydrolase family of pyrophosphatases. NUDT21 localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 being the smaller subunit of the complex, is present in all heterodimer combinations. NUDT21 has a vital role in pre-mRNA 3' cleavage and polyadenylation processing.
NUDT21 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 247 amino acids (1-227 a.a.) and having a molecular mass of 28.3kDa. The NUDT21 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CFIM25: Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export. Belongs to the Nudix hydrolase family. CPSF5 subfamily. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: nucleoplasm; centrosome; nucleus; paraspeckles; mRNA cleavage factor complex Molecular Function:mRNA binding; protein binding; protein homodimerization activity; hydrolase activity; RNA binding; histone deacetylase binding; AU-rich element binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; RNA splicing; gene expression; mRNA polyadenylation; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; protein tetramerization |
| NCBI Summary: | The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43809 |
| NCBI GenInfo Identifier: | 74735411 |
| NCBI Gene ID: | 11051 |
| NCBI Accession: | O43809.1 |
| UniProt Secondary Accession: | O43809,Q6IB85, Q6NE84, |
| UniProt Related Accession: | O43809 |
| Molecular Weight: | 227 |
| NCBI Full Name: | Cleavage and polyadenylation specificity factor subunit 5 |
| NCBI Synonym Full Names: | nudix (nucleoside diphosphate linked moiety X)-type motif 21 |
| NCBI Official Symbol: | NUDT21 |
| NCBI Official Synonym Symbols: | CPSF5; CFIM25 |
| NCBI Protein Information: | cleavage and polyadenylation specificity factor subunit 5; nudix motif 21; CPSF 25 kDa subunit; pre-mRNA cleavage factor Im (25kD); cleavage factor Im complex 25 kDa subunit; pre-mRNA cleavage factor Im, 25kD subunit; pre-mRNA cleavage factor Im 25 kDa subunit; nucleoside diphosphate-linked moiety X motif 21; cleavage and polyadenylation specific factor 5, 25 kDa; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specificity factor 25 kDa subunit |
| UniProt Protein Name: | Cleavage and polyadenylation specificity factor subunit 5 |
| UniProt Synonym Protein Names: | Cleavage and polyadenylation specificity factor 25 kDa subunit; CFIm25; CPSF 25 kDa subunit; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Pre-mRNA cleavage factor Im 25 kDa subunit |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT21 |
| UniProt Entry Name: | CPSF5_HUMAN |
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