Human NPL Recombinant Protein (RPPB2033)

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SKU:
RPPB2033
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
NPL
Synonyms:
N-acetylneuraminate lyase
Synonyms:
NALase
Synonyms:
N-acetylneuraminate pyruvate-lyase
Synonyms:
N-acetylneuraminic acid aldolase
Source:
Escherichia Coli
Uniprot:
Q9BXD5
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Description

Product Name:Human NPL Recombinant Protein
Product Code:RPPB2033
Size:20µg
Species:Human
Target:NPL
Synonyms:N-acetylneuraminate lyase, NALase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase, NPL, C1orf13, NAL, C112, NPL1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NPL protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEW VTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLD GILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQ RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS

N-acetylneuraminate lyase (NPL) is an enzyme which catalyzes the chemical reaction (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate). NPL is a member of a family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NPL participates in amino sugars metabolism.

NPL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (1-320 a.a.) and having a molecular mass of 37.3kDa.NPL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NPL: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Belongs to the DHDPS family. NanA subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.1.3.3; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Lyase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytosol

Molecular Function:N-acetylneuraminate lyase activity; protein binding

Biological Process: N-acetylneuraminate catabolic process

NCBI Summary:This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:Q9BXD5
NCBI GenInfo Identifier:74752428
NCBI Gene ID:80896
NCBI Accession:Q9BXD5.1
UniProt Secondary Accession:Q9BXD5,Q4G0Q8, Q4G0Z2, Q64L88, Q6PEL0, B2R839,
UniProt Related Accession:Q9BXD5
Molecular Weight:12,201 Da
NCBI Full Name:N-acetylneuraminate lyase
NCBI Synonym Full Names:N-acetylneuraminate pyruvate lyase
NCBI Official Symbol:NPL  
NCBI Official Synonym Symbols:NAL; C112; NPL1; C1orf13  
NCBI Protein Information:N-acetylneuraminate lyase
UniProt Protein Name:N-acetylneuraminate lyase
UniProt Synonym Protein Names:N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialate-pyruvate lyase; Sialic acid aldolase; Sialic acid lyase
Protein Family:NPL4-like protein
UniProt Gene Name:NPL  
UniProt Entry Name:NPL_HUMAN
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