Human NENF Recombinant Protein (RPPB0782)

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SKU:
RPPB0782
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
NENF
Synonyms:
Neudesin
Synonyms:
Cell immortalization-related protein 2
Synonyms:
Neuron-derived neurotrophic factor
Synonyms:
Secreted protein of unknown function
Source:
Escherichia Coli
Uniprot:
Q9UMX5
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Description

Product Name:Human NENF Recombinant Protein
Product Code:RPPB0782
Size:10µg
Species:Human
Target:NENF
Synonyms:Neudesin, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor, Secreted protein of unknown function, SPUF protein, NENF, CIR2, SPUF, SCIRP10.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:NENF was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. NENF is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHASGQTPRPAERG PPVRLFTEEE LARYGGEEED QPIYLAVKGV VFDVTSGKEF YGRGAPYNAL TGKDSTRGVA KMSLDPADLT HDTTGLTAKE LEALDEVFTK VYKAKYPIVG YTARRILNED GSPNLDFKPE DQPHFDIKDE F

Neudesin Neurotrophic Factor (NENF) is a member of the cytochrome b5 family, MAPR subfamily. NENF contains 1 cytochrome b5 heme-binding domain. NENF exhibits neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. NENF doesn’t have mitogenic activity in primary cultured astrocytes. NENF may play a part in neuronal differentiation and may have a transient influence on neural cell proliferation in neural precursor cells. NENF neurotrophic activity is increased by binding to heme. NENF is up-regulated in immortal cells and induced in estrogen receptor positive breast cancer expressing progesterone receptor.

NENF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 32-172) containing 151 amino acids including a 10 a.a N-terminal His tag and having a molecular mass of 16.9kDa.

UniProt Protein Function:NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 1q32.3Cellular Component: endomembrane system; extracellular space; membraneMolecular Function: growth factor activity; metal ion bindingBiological Process: negative regulation of appetite; positive regulation of MAPK cascade
UniProt Protein Details:
NCBI Summary:This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]
UniProt Code:Q9UMX5
NCBI GenInfo Identifier:7019545
NCBI Gene ID:29937
NCBI Accession:NP_037481.1
UniProt Secondary Accession:Q9UMX5,Q53FZ6, Q5TM90, A1KYQ8
UniProt Related Accession:Q9UMX5
Molecular Weight:18,856 Da
NCBI Full Name:neudesin
NCBI Synonym Full Names:neudesin neurotrophic factor
NCBI Official Symbol:NENF  
NCBI Official Synonym Symbols:CIR2; SPUF; SCIRP10  
NCBI Protein Information:neudesin
UniProt Protein Name:Neudesin
UniProt Synonym Protein Names:Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47
Protein Family:Neudesin
UniProt Gene Name:NENF  
UniProt Entry Name:
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