Human MYL12B Recombinant Protein (RPPB4032)
- SKU:
- RPPB4032
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- MYL12B
- Synonyms:
- Myosin light chain 12B regulatory
- Myosin regulatory light chain 2-B smooth muscle isoform
- Myosin regulatory light chain 20 kDa
- Myosin regulatory light chain MRLC2
- Source:
- Escherichia Coli
- Uniprot:
- O14950
Description
| Product Name: | Human MYL12B Recombinant Protein |
| Product Code: | RPPB4032 |
| Size: | 20µg |
| Species: | Human |
| Target: | MYL12B |
| Synonyms: | Myosin light chain 12B regulatory, Myosin regulatory light chain 2-B smooth muscle isoform, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, MYLC2B, MRLC2, MLC-2A, MLC-B, SHUJUN-1 MLC20. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MYL12B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 2mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD |
Myosin is composed of 2 nonphosphorylatable alkali light chains, 2 heavy chains and 2 phosphorylatable regulatory light chains. MYL12B is a hexameric ATPase cellular motor protein which controls contraction in smooth muscle and non-muscle cells through phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains catalyzed by MLCK in the presence of calcium and calmodulin, rises Actin-activated myosin ATPase activity, thus regulating contractile activity.
MYL12B Human Recombinant produced in E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.3kDa.MYL12B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains. |
| UniProt Protein Details: | Protein type:Contractile Chromosomal Location of Human Ortholog: 18p11.31 Cellular Component: cytosol Molecular Function:protein binding Biological Process: muscle contraction |
| NCBI Summary: | This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014] |
| UniProt Code: | O14950 |
| NCBI GenInfo Identifier: | 205829213 |
| NCBI Gene ID: | 10627 |
| NCBI Accession: | O14950.2 |
| UniProt Secondary Accession: | O14950,Q13182, Q7Z5Z4, D3DUH6, |
| UniProt Related Accession: | P19105 |
| Molecular Weight: | 19,779 Da |
| NCBI Full Name: | Myosin regulatory light chain 12B |
| NCBI Synonym Full Names: | myosin light chain 12A |
| NCBI Official Symbol: | MYL12A |
| NCBI Official Synonym Symbols: | MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24 |
| NCBI Protein Information: | myosin regulatory light chain 12A |
| UniProt Protein Name: | Myosin regulatory light chain 12B |
| UniProt Synonym Protein Names: | MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1 |
| Protein Family: | Myosin regulatory |
| UniProt Gene Name: | MYL12B |
| UniProt Entry Name: | ML12B_HUMAN |
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