The Human MUC13 (Mucin 13) CLIA Kit is specifically designed for the precise measurement of Mucin 13 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Mucin 13 is a mucin protein that plays a critical role in cell adhesion, signaling, and immune response.
Abnormalities in Mucin 13 expression have been linked to various cancers and inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human MUC13 CLIA Kit is a reliable tool for researchers looking to investigate the role of Mucin 13 in disease pathology and therapeutic development.
Product Name:
Human MUC13 (Mucin 13) CLIA Kit
SKU:
HUES00406
Target:
Human MUC13 (Mucin 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC13. You can calculate the concentration of Human MUC13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
90-103
97-115
Average (%)
92
95
105
1:4
Range (%)
100-119
96-113
99-111
Average (%)
108
103
105
1:8
Range (%)
95-108
83-96
98-111
Average (%)
101
90
104
1:16
Range (%)
101-116
96-111
92-107
Average (%)
107
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
103
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
101-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.49
318.77
718.85
102.12
324.4
766.54
Standard deviation
10.31
28.78
56.07
8.48
24.3
87.54
C V (%)
9.77
9.03
7.8
8.3
7.49
11.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC13 in samples. No significant cross-reactivity or interference between Human MUC13 and analogues was observed.
