Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
The Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit is a reliable tool for the quantitative measurement of MMP-8 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for your research needs.MMP-8 is a key enzyme that plays a crucial role in the breakdown of extracellular matrix proteins, leading to tissue remodeling and turnover. Its dysregulation has been implicated in various pathological conditions, including inflammation, wound healing, and cancer progression.
Therefore, the detection of MMP-8 levels can provide valuable insights into these disease processes and facilitate the development of targeted therapies.By using the Human MMP-8 ELISA Kit, researchers can gain a better understanding of MMP-8 expression levels in different samples and investigate its potential as a biomarker for disease diagnosis and prognosis. This kit is a valuable addition to any laboratory conducting research in the fields of cancer biology, inflammation, and tissue regeneration.
Product Name:
Human MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
SKU:
HUES02485
Target:
Human MMP-8 (Matrix Metalloproteinase 8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-8. You can calculate the concentration of Human MMP-8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-112
92-109
91-105
Average (%)
106
100
97
1:4
Range (%)
94-110
86-102
95-112
Average (%)
102
93
102
1:8
Range (%)
98-112
86-99
96-113
Average (%)
106
93
104
1:16
Range (%)
98-110
87-99
94-112
Average (%)
104
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
4.4
0.5
1.2
4.1
Standard deviation
0.03
0.06
0.23
0.03
0.06
0.17
C V (%)
6
5.0
5.23
6.0
5.0
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-8 in samples. No significant cross-reactivity or interference between Human MMP-8 and analogues was observed.
