Human MME Recombinant Protein (RPPB1949)

(No reviews yet) Write a Review

제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.

주문
SKU:
RPPB1949
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
MME
Synonyms:
Membrane Metalloendopeptidase
Synonyms:
Common Acute Lymphocytic Leukemia Antigen
Synonyms:
Neutral Endopeptidase 2411
Synonyms:
Skin Fibroblast Elastase
Source:
Sf9 Insect cells
Uniprot:
P08473
€0.00
Frequently bought together:

Description

Product Name:Human MME Recombinant Protein
Product Code:RPPB1949
Size:10µg
Species:Human
Target:MME
Synonyms:Membrane Metalloendopeptidase, Common Acute Lymphocytic Leukemia Antigen, Neutral Endopeptidase 24.11, Skin Fibroblast Elastase, Neutral Endopeptidase, Atriopeptidase, Enkephalinase, EC 3.4.24.11, Neprilysin, CALLA, NEP, SFE,Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10), Membrane Metallo-Endopeptidase Variant 1, Membrane Metallo-Endopeptidase Variant 2, Neprilysin-390, Neprilysin-411, CD10 Antigen, EC 3.4.24, CMT2T, SCA43, CD10, EPN, MME.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MME solution (1mg/ml) contains 10% Glycerol, 20 mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPYDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIP ETSSRYGNFD ILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLL KLLPDIYGWP VATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIH IDQPRLGLPS RDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVME LEKEIANATA KPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISIT NEEDVVVYAP EYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKAL YGTTSETATW RRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDL TWMDAETKKR AEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFS QSKQLKKLRE KVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGI GMVIGHEITH GFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQH LNGINTLGEN IADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTY RPEYAVNSIK TDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VWHHHHHH
Biological Activity:Specific activity > 5,000 pmol/min/ug. One unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute, at pH 8.8 at 25C?.

Neutral endopeptidase (NEP) is an enzyme located in the cell membrane (bound to it) that is able to dissolve biologically active proteins and is expressed on the surface of lymphoid progenitors, human podocytes, syncytiotrophoblastic cells, and many other epithelial cells including polymorphonuclear leukocytes.

MME Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 708 amino acids (52-750 aa) and having a molecular mass of 80.9kDa.MME is fused to a 9 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques. 

UniProt Protein Function:CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.
UniProt Protein Details:

Protein type:EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; plasma membrane; synapse; synaptic vesicle

Molecular Function:endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: angiotensin maturation; beta-amyloid metabolic process; creatinine metabolic process; kidney development; peptide metabolic process; proteolysis; sensory perception of pain

Disease: Membranous Nephropathy, Susceptibility To

NCBI Summary:This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]
UniProt Code:P08473
NCBI GenInfo Identifier:128062
NCBI Gene ID:4311
NCBI Accession:P08473.2
UniProt Secondary Accession:P08473,Q3MIX4, A8K6U6, D3DNJ9,
UniProt Related Accession:P08473
Molecular Weight:85,514 Da
NCBI Full Name:Neprilysin
NCBI Synonym Full Names:membrane metallo-endopeptidase
NCBI Official Symbol:MME  
NCBI Official Synonym Symbols:NEP; SFE; CD10; CALLA; CMT2T  
NCBI Protein Information:neprilysin
UniProt Protein Name:Neprilysin
UniProt Synonym Protein Names:Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD_antigen: CD10
Protein Family:Neprilysin
UniProt Gene Name:MME  
UniProt Entry Name:NEP_HUMAN
View AllClose

Related Products