Human MAFK Recombinant Protein (RPPB3909)

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SKU:
RPPB3909
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
MAFK
Synonyms:
NFE2U
Synonyms:
P18
Synonyms:
Erythroid transcription factor NF-E2 p18 subunit
Synonyms:
MAFK
Source:
Escherichia Coli
Uniprot:
O60675
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Description

Product Name:Human MAFK Recombinant Protein
Product Code:RPPB3909
Size:20µg
Species:Human
Target:MAFK
Synonyms:NFE2U, P18, Erythroid transcription factor NF-E2 p18 subunit, MAFK, Transcription factor MafK.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:MAFK protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS

MAFK is a member of the bZIP family and Maf subfamily. Members from Maf family of basic region/leucine zipper (bZIP) transcription factors influence transcription in a positive or negative way, depending on their protein associate and the context of the target promoter. Small Mafs act as transcriptional repressors when they dimerize among themselves. Yet, they seem to function as transcriptional activators by dimerizing with other basic-zipper proteins and employing them to specific DNA-binding sites.

MAFK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 19.7kDa.MAFK is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MAFK: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Belongs to the bZIP family. Maf subfamily.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: nucleoplasm

Molecular Function:transcription factor activity

Biological Process: nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; blood coagulation

NCBI Summary:The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]
UniProt Code:O60675
NCBI GenInfo Identifier:4505075
NCBI Gene ID:7975
NCBI Accession:NP_002351.1
UniProt Secondary Accession:O60675,A4D214,
UniProt Related Accession:O60675
Molecular Weight:17,523 Da
NCBI Full Name:transcription factor MafK
NCBI Synonym Full Names:v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K
NCBI Official Symbol:MAFK  
NCBI Official Synonym Symbols:P18; NFE2U  
NCBI Protein Information:transcription factor MafK; nuclear factor erythroid-2, ubiquitous (p18); basic-leucine zipper transcription factor MafK; erythroid transcription factor NF-E2 p18 subunit; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K; v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K
UniProt Protein Name:Transcription factor MafK
UniProt Synonym Protein Names:Erythroid transcription factor NF-E2 p18 subunit
Protein Family:Transcription factor
UniProt Gene Name:MAFK  
UniProt Entry Name:MAFK_HUMAN
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