The Human Lp(a) (Lipoprotein(a)) CLIA Kit is specifically designed for the accurate and efficient detection of Lipoprotein(a) levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Lipoprotein(a) is a unique lipoprotein that plays a significant role in cardiovascular disease and atherosclerosis. Elevated levels of Lipoprotein(a) have been linked to an increased risk of heart attacks and strokes, making it a valuable biomarker for studying and monitoring cardiovascular health.
With this CLIA kit, researchers can easily measure Lipoprotein(a) levels in biological samples, enabling them to further investigate the role of Lipoprotein(a) in cardiovascular diseases and develop potential therapeutic interventions. Trust the Human Lp(a) (Lipoprotein(a)) CLIA Kit for accurate and reliable results in your research endeavors.
Product Name:
Human LP-a (Lipoprotein a) CLIA Kit
SKU:
HUES00140
Target:
Human LP-a (Lipoprotein a)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LP-a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LP-a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LP-a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LP-a. You can calculate the concentration of Human LP-a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
90-100
87-99
Average (%)
103
95
94
1:4
Range (%)
88-103
99-117
99-116
Average (%)
95
107
106
1:8
Range (%)
85-100
90-103
89-104
Average (%)
92
96
96
1:16
Range (%)
96-107
83-98
93-105
Average (%)
101
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
103
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.46
609.33
1339.18
195.92
667.66
1376.47
Standard deviation
19.89
49.11
137.53
17.81
75.58
107.23
C V (%)
9.68
8.06
10.27
9.09
11.32
7.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LP-a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LP-a in samples. No significant cross-reactivity or interference between Human LP-a and analogues was observed.
