Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type I II multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.
Product Name:
Human LIMP-2/LIMPII Recombinant Protein (RPES4032)
Product Code:
RPES4032
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Lysosome Membrane Protein 2, 85 kDa Lysosomal Membrane Sialoglycoprotein, LGP85, CD36 Antigen-Like 2, Lysosome Membrane Protein II, LIMP II, Scavenger Receptor Class B Member 2, CD36, SCARB2, CD36L2, LIMPII,AMRF,CD36L2,EPM4,HLGP85,LGP85,SR-BII
Accession:
NP_005497.1
Sequence:
Arg 27-Thr 432
Fusion tag:
C-His & Fc
Activity:
Measured by its ability to bind recombinant human RSPO1 in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SCARB2 (NP_005497.1) extracellular domain (Arg 27-Thr 432) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
74.4 kDa
AP Mol Mass:
110-115 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
