Kelch-like ECH-associated protein 1; also known as cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 and INRF2; is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions; KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome; thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Product Name:
Human KEAP1/INRF2 Recombinant Protein (RPES4295)
Product Code:
RPES4295
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
INRF2,KEAP-1,KLHL19
Accession:
Q14145
Sequence:
Gln2-Cys624
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
