Human IYD Recombinant Protein (RPPB1854)

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SKU:
RPPB1854
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
IYD
Synonyms:
Iodotyrosine dehalogenase 1
Synonyms:
IYD-1
Synonyms:
Iodotyrosine Deiodinase
Synonyms:
IYD
Source:
Escherichia Coli
Uniprot:
Q6PHW0
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Description

Product Name:Human IYD Recombinant Protein
Product Code:RPPB1854
Size:20µg
Species:Human
Target:IYD
Synonyms:Iodotyrosine dehalogenase 1, IYD-1, Iodotyrosine Deiodinase, IYD, C6orf71, DEHAL1, iodotyrosine dehalogenase 1 isoform 3, dJ422F24.1, TDH4.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:IYD protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSDRSMEKK KGEPRTRAEA RPWVDEDLKD SSDLHQAEED ADEWQESEEN VEHIPFSHNH YPEKEMVKRS QEFYELLNKR RSVRFISNEQ VPMEVIDNVI RTAGTAPSGA HTEPWTFVVV KDPDVKHKIR KIIEEEEEIN YMKRMGHRWV TDLKKLRTNW IKEYLDTAPI LILIFKQVHG FAANGKKKVH YYNE

IYD is an enzyme that catalyzes the oxidative NADPH-dependent deiodination of both mono- and diiodotyrosine although acts more efficiently on monoiodotyrosine. The N-terminus of Iodotyrosine Deiodinase , also known as IYD, plays a role as a membrane anchor. IYD acts during the hydrolysis of thyroglobulin to liberate iodide, which then reenter the hormone-producing pathways.

IYD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 214 amino acids (24-214 a.a) and having a molecular mass of 25.1kDa.IYD is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:IYD: Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine. Defects in IYD are the cause of thyroid dyshormonogenesis 4 (TDH4). A disorder due to thyroid dyshormonogenesis, causing severe hypothyroidism, goiter, excessive levels of iodotyrosine in serum and urine, and variable mental deficits derived from unrecognized and untreated hypothyroidism. Belongs to the nitroreductase family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; EC 1.22.1.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function:iodide peroxidase activity; oxidoreductase activity

Biological Process: thyroid hormone generation

Disease: Thyroid Dyshormonogenesis 4

NCBI Summary:This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Code:Q6PHW0
NCBI GenInfo Identifier:33989988
NCBI Gene ID:389434
NCBI Accession:BC056253
UniProt Secondary Accession:Q6PHW0,Q2VPW0, Q2VPW1, Q5F1L5, Q5F1L6, Q5THM4, Q6ZP69 Q7Z7D7, Q7Z7D8, C9JFW2,
UniProt Related Accession:Q6PHW0
Molecular Weight:22,460 Da
NCBI Full Name:Homo sapiens iodotyrosine deiodinase, mRNA
NCBI Synonym Full Names:iodotyrosine deiodinase
NCBI Official Symbol:IYD  
NCBI Official Synonym Symbols:TDH4; DEHAL1; C6orf71; dJ422F24.1  
NCBI Protein Information:iodotyrosine dehalogenase 1; IYD-1
UniProt Protein Name:Iodotyrosine dehalogenase 1
Protein Family:Iodotyrosine deiodinase
UniProt Gene Name:IYD  
UniProt Entry Name:IYD1_HUMAN
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