Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.
Product Name:
Human ILRAcP/ILR3 Recombinant Protein (RPES1834)
Product Code:
RPES1834
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
C3orf13,IL-1RAcP,IL1R3
Accession:
NP_002173.1
Sequence:
Met 1-Glu 359
Fusion tag:
C-His & Fc
Activity:
Measured by its ability to bind with biotinylated human IL1R2-Hisin a functional ELISA.2. Immobilized human IL1R3-Fch at 10 µg/mL (100ul/well) can bind biotinylated human IL1B-His. The EC50 of biotinylated human IL1B-His is 0.11-0.25 µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL1R3 (NP_002173.1) extracellular domain (Met 1-Glu 359) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
67.3 kDa
AP Mol Mass:
75-85 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.