Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
Product Name:
Human IL3RA/CD123 Recombinant Protein (RPES3931)
Product Code:
RPES3931
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD123,hIL-3Ra,IL3R,IL3RAY,IL3RX,IL3RY
Accession:
NP_002174.1
Sequence:
Met 1-Arg 305
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL3 at 20 µg/ml (100 ?l/well) can bind biotinylated human IL3RA with a linear ranger of 0.32-1.6 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
34.5 kDa
AP Mol Mass:
50-60 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
