The Human IL-23 ELISA Kit is a powerful tool for the precise measurement of IL-23 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IL-23 is a key cytokine involved in the regulation of immune responses, particularly in the development of inflammatory and autoimmune diseases.
Elevated levels of IL-23 have been linked to conditions such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease, making it a valuable biomarker for studying these disorders and developing targeted therapies.With its reliable performance and user-friendly protocol, the Human IL-23 ELISA Kit from AssayGenie is an essential tool for researchers seeking to explore the role of IL-23 in health and disease.
Product Name:
Human IL-23 ELISA Kit
SKU:
HUES01393
Target:
Human IL-23
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-23. You can calculate the concentration of Human IL-23 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
95-109
85-99
Average (%)
99
102
91
1:4
Range (%)
93-104
84-96
86-102
Average (%)
98
90
93
1:8
Range (%)
91-106
83-94
87-99
Average (%)
99
89
94
1:16
Range (%)
88-103
84-94
80-92
Average (%)
95
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
136.57
343.26
1072.26
129.52
356.17
1144.3
Standard deviation
7.2
15.55
35.49
8.48
20.02
54.47
C V (%)
5.27
4.53
3.31
6.55
5.62
4.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-23 in samples. No significant cross-reactivity or interference between Human IL-23 and analogues was observed.
