The Human IL11 ELISA Kit is a high-quality product designed for the precise measurement of IL11 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it perfect for a variety of research purposes.IL11 is a key cytokine involved in various biological processes such as inflammation, cell growth, and immune response regulation. Its dysregulation has been linked to several diseases including cancer, inflammatory disorders, and autoimmune conditions, highlighting its importance as a target for study and therapeutic development.
By utilizing the Human IL11 ELISA Kit, researchers can gain valuable insights into the role of IL11 in disease pathogenesis and potentially discover new treatment strategies. Don't miss out on the opportunity to accelerate your research with this cutting-edge ELISA kit.
Product Name:
Human IL-11 ELISA Kit
SKU:
HUES03391
Target:
Human IL-11
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-11. You can calculate the concentration of Human IL-11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-109
93-107
93-106
Average (%)
99
101
98
1:4
Range (%)
91-104
87-98
85-99
Average (%)
97
92
92
1:8
Range (%)
88-103
87-100
81-91
Average (%)
95
92
87
1:16
Range (%)
90-102
83-98
88-102
Average (%)
95
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.84
169.33
941.42
115.22
161.16
978.65
Standard deviation
6.66
10.08
39.16
7.24
8.06
47.17
C V (%)
6.18
5.95
4.16
6.28
5.0
4.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-11 in samples. No significant cross-reactivity or interference between Human IL-11 and analogues was observed.
