Human Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) ELISA Kit
The Human Hydroxymethylglutaryl-Coenzyme A Synthase (HMGCS) ELISA Kit is a powerful tool for the precise measurement of HMGCS levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.HMGCS is a key enzyme in the biosynthesis of cholesterol and other essential molecules in the body, making it a critical target for studying metabolic disorders, cardiovascular diseases, and other related conditions.
By accurately measuring HMGCS levels, researchers can gain valuable insights into these diseases and potentially develop novel treatment strategies.Don't miss out on the opportunity to explore the intricate role of HMGCS in human health and disease with the Human HMGCS ELISA Kit from Assay Genie. Order yours today and take your research to the next level.
Product Name:
Human Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS) ELISA Kit
Product Code:
HUDL01344
Size:
96 Assays
Target:
HMGCS
Synonyms:
HMGCS1, 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble, HMG-CoA Synthase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
