Human Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1) ELISA Kit
The Human Heparan Sulfate 6-O-Sulfotransferase 1 (HS6ST1) ELISA Kit is a powerful tool for researchers studying the role of HS6ST1 in various biological processes. This kit allows for the accurate measurement of HS6ST1 levels in human serum, plasma, and cell culture supernatants with high sensitivity and specificity.HS6ST1 is an important enzyme involved in the sulfation of heparan sulfate, a key component of the extracellular matrix that plays a critical role in cell signaling, tissue development, and inflammation.
Dysregulation of HS6ST1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.By accurately quantifying HS6ST1 levels, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets. The Human HS6ST1 ELISA Kit offers a reliable and reproducible method for studying HS6ST1 biology and its implications in health and disease.
Product Name:
Human Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1) ELISA Kit
Product Code:
HUDL01367
Size:
96 Assays
Target:
HS6ST1
Synonyms:
HS6ST
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
