Human Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) ELISA Kit
The Human Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1) ELISA Kit is specifically designed for the precise quantification of HS2ST1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HS2ST1 is a key enzyme involved in the biosynthesis of heparan sulfate, a critical component of the extracellular matrix and cell surface. Aberrant expression of HS2ST1 has been linked to various diseases, including cancer, inflammation, and developmental disorders.
Detecting HS2ST1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human HS2ST1 ELISA Kit is a reliable tool for scientists and researchers studying heparan sulfate biology, as well as those investigating the role of HS2ST1 in various pathological conditions. It offers a convenient and efficient method for measuring HS2ST1 levels, contributing to the advancement of biomedical research and drug discovery efforts.
Product Name:
Human Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) ELISA Kit
Product Code:
HUDL01366
Size:
96 Assays
Target:
HS2ST1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
