The Human Hemoglobin Gamma-1 (HBG1) ELISA Kit is specifically designed for the quantitative measurement of HBG1 levels in human serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for various research applications.HBG1 is a vital component of hemoglobin, the protein responsible for oxygen transport in the blood. Abnormal levels of HBG1 have been linked to various hematological disorders, such as sickle cell disease and thalassemia, making it a crucial biomarker for studying and diagnosing these conditions.
By utilizing the Human HBG1 ELISA Kit, researchers can gain valuable insights into the role of HBG1 in health and disease, paving the way for the development of innovative diagnostic tools and therapeutic interventions.
Product Name:
Human Hemoglobin Gamma 1 (HBg1) ELISA Kit
Product Code:
HUDL01307
Size:
96 Assays
Target:
HBg1
Synonyms:
HB-G1, HBGA, HBGR, HSGGL1, Gamma-1-globin
Detection Method:
Competition
Range:
468.75-30,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
