Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit
The Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit is specifically designed for the precise measurement of HDGF levels in human samples such as serum, plasma, and cell culture supernatants. With unparalleled sensitivity and accuracy, this kit delivers consistent and dependable results, making it indispensable for various research studies.HDGF is a vital growth factor that plays a critical role in promoting cell growth and proliferation.
Its involvement in various physiological processes makes it a key player in the study of conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders. By accurately measuring HDGF levels, researchers can gain valuable insights into disease progression and develop potential therapeutic interventions.
Product Name:
Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit
SKU:
HUES03616
Target:
Human HDGF (Hepatoma Derived Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HDGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HDGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HDGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HDGF. You can calculate the concentration of Human HDGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-104
96-111
Average (%)
92
98
101
1:4
Range (%)
91-103
80-94
86-100
Average (%)
98
86
92
1:8
Range (%)
93-108
86-96
87-104
Average (%)
98
91
95
1:16
Range (%)
91-102
84-93
88-101
Average (%)
96
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
1.85
9.94
0.92
1.96
10.6
Standard deviation
0.07
0.07
0.31
0.06
0.09
0.56
C V (%)
7.14
3.78
3.12
6.52
4.59
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HDGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HDGF in samples. No significant cross-reactivity or interference between Human HDGF and analogues was observed.
