The Human Gastric Intrinsic Factor (GIF) ELISA Kit is a cutting-edge tool for the precise measurement of gastric intrinsic factor levels in human samples including serum and plasma. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Gastric intrinsic factor is a vital protein essential for the absorption of vitamin B12 in the stomach. Deficiencies in intrinsic factor can lead to serious health issues such as pernicious anemia.
By accurately measuring intrinsic factor levels, researchers can gain valuable insights into gastric health and diagnose potential deficiencies early on, allowing for timely intervention and treatment.Whether investigating the role of gastric intrinsic factor in digestive disorders or evaluating its potential as a biomarker for certain conditions, the Human Gastric Intrinsic Factor (GIF) ELISA Kit is an invaluable asset for advancing scientific knowledge and improving patient care.
Product Name:
Human Gastric Intrinsic Factor (GIF) ELISA Kit
Product Code:
HUDL01188
Size:
96 Assays
Target:
GIF
Synonyms:
TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis
Detection Method:
Competition
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
