The Human FPA (Fibrinopeptide A) ELISA Kit is specifically designed for the precise and reliable detection of fibrinopeptide A levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are suitable for a variety of research purposes.
Fibrinopeptide A is a vital component involved in the blood coagulation process, serving as a marker for various cardiovascular and inflammatory conditions. By measuring FPA levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify novel therapeutic targets.
Product Name:
Human FPA (Fibrinopeptide A) ELISA Kit
SKU:
HUES03090
Target:
Human FPA (Fibrinopeptide A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FPA. You can calculate the concentration of Human FPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
97-111
89-103
Average (%)
90
103
95
1:4
Range (%)
90-102
86-99
81-94
Average (%)
95
91
87
1:8
Range (%)
92-107
81-94
85-100
Average (%)
98
86
91
1:16
Range (%)
93-108
84-96
88-101
Average (%)
100
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
98
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.47
15.11
49.51
5.59
16.49
52.56
Standard deviation
0.3
0.89
1.88
0.37
0.97
2.83
C V (%)
5.48
5.89
3.8
6.62
5.88
5.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FPA in samples. No significant cross-reactivity or interference between Human FPA and analogues was observed.
