The Human FBN1 (Fibrillin-1) ELISA Kit is specifically designed for the precise measurement of fibrillin-1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Fibrillin-1 is a critical component of the extracellular matrix and plays a vital role in maintaining tissue integrity and elasticity. Mutations in the FBN1 gene have been associated with various genetic disorders, including Marfan syndrome and congenital contractural arachnodactyly.
Monitoring fibrillin-1 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic interventions.With its reliable performance and precise detection capabilities, the Human FBN1 ELISA Kit is an indispensable tool for researchers studying fibrillin-1 biology and its implications in health and disease. Invest in this kit today to advance your research efforts and contribute to scientific advancements in the field of genetics and connective tissue disorders.
Product Name:
Human FBN1 (Fibrillin 1) ELISA Kit
SKU:
HUES03150
Target:
Human FBN1 (Fibrillin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FBN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FBN1. You can calculate the concentration of Human FBN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
97-109
87-103
Average (%)
95
103
94
1:4
Range (%)
94-106
81-93
84-97
Average (%)
100
86
89
1:8
Range (%)
87-98
81-94
87-101
Average (%)
92
87
94
1:16
Range (%)
86-99
79-89
82-95
Average (%)
92
85
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
1.86
7.7
1.03
2.01
8.45
Standard deviation
0.07
0.08
0.34
0.07
0.12
0.3
C V (%)
6.67
4.3
4.42
6.8
5.97
3.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FBN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBN1 in samples. No significant cross-reactivity or interference between Human FBN1 and analogues was observed.
