Human EEF2K Recombinant Protein (RPPB2501)

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SKU:
RPPB2501
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
EEF2K
Synonyms:
Eukaryotic Elongation Factor 2 Kinase
Synonyms:
Calcium/Calmodulin-DependentEukaryotic Elongation Factor 2 Kinase
Synonyms:
EEF-2 Kinase
Synonyms:
EC 271120
Source:
Escherichia Coli
Uniprot:
O00418
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Description

Product Name:Human EEF2K Recombinant Protein
Product Code:RPPB2501
Size:10µg
Species:Human
Target:EEF2K
Synonyms:Eukaryotic Elongation Factor 2 Kinase, Calcium/Calmodulin-DependentEukaryotic Elongation Factor 2 Kinase, EEF-2 Kinase, EC 2.7.11.20, EEF-2K, Calcium/Calmodulin-DependentEukaryotic Elongation Factor-2 Kinase, Calmodulin-Dependent Protein Kinase III,Eukaroytic Elongation Factor 2 Kinase, Eukaryotic Elongation Factor-2 Kinase, ElongationFactor-2 Kinase, EC 2.7.11, HSU93850, Eukaryotic elongation factor 2 kinase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EEF2K protein solution (0.25 mg/ml) containing Phosphatebuffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPSSNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRYNAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLHAQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQVDIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQGVGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVNQNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSDVTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPREHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKKIGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAIVGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEALHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAAEAAMEAMKGR LANQYYQKAE EAWAQMEE

Eukaryotic elongation factor-2 kinase (EEF2K) is threonine kinase whichregulates protein synthesis by means of controlling the rate of peptide chainelongation. Upon activation through a diversity of upstream kinases includingAMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a sole site,renders it unable to bind ribosomes and therefore inactive.  In turn, the rate ofprotein synthesis is being reduced.

EEF2K Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 748 amino acids (1-725 a.a) andhaving a molecular mass of 84.6kDa.EEF2K is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Translation; Kinase, protein; Protein kinase, atypical; EC 2.7.11.20; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function:calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway

NCBI Summary:This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]
UniProt Code:O00418
NCBI GenInfo Identifier:254763289
NCBI Gene ID:29904
NCBI Accession:O00418.2
UniProt Secondary Accession:O00418,Q8N588,
UniProt Related Accession:O00418
Molecular Weight:725
NCBI Full Name:Eukaryotic elongation factor 2 kinase
NCBI Synonym Full Names:eukaryotic elongation factor 2 kinase
NCBI Official Symbol:EEF2K  
NCBI Official Synonym Symbols:eEF-2K; HSU93850  
NCBI Protein Information:eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
UniProt Protein Name:Eukaryotic elongation factor 2 kinase
UniProt Synonym Protein Names:Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Protein Family:Eukaryotic elongation factor 2 kinase
UniProt Gene Name:EEF2K  
UniProt Entry Name:EF2K_HUMAN
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