Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. In mammals, four Notch homologs (Notch 1 to4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 shares 86% amino acid sequence identity. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteinerich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ ? secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively.
Product Name:
Human DLL4 Recombinant Protein (RPES3113)
Product Code:
RPES3113
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Delta-like protein 4, Drosophila Delta homolog 4, Delta4, DLL4
Accession:
Q9NR61
Sequence:
Ser27-Pro524
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human DLL4 is produced by our Mammalian expression system and the target gene encoding Ser27-Pro524 is expressed with a 10His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
55.7 kDa
AP Mol Mass:
66 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
