Dickkopf-related protein 2 (DKK2), is a member of the dickkopf family. DKK2 is secreted protein which contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Product Name:
Human DKK2 Recombinant Protein (RPES2309)
Product Code:
RPES2309
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2
Accession:
Q9UBU2
Sequence:
Asp251-Ile526
Fusion tag:
N-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Dickkopf-related protein 2 is produced by our Mammalian expression system and the target gene encoding Asp251-Ile526 is expressed with a Fc tag at the N-terminus, 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
58.9 kDa
AP Mol Mass:
55 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
